阿特拉津和毒死蜱对东北小鲵蝌蚪氧化应激的影响

为了研究阿特拉津和毒死蜱单一及联合暴露对东北小鲵(Hynobius leechii)蝌蚪抗氧化酶活性的影响,实验选择不同浓度、不同时间点阿特拉津和毒死蜱单一及联合暴露对东北小鲵蝌蚪超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)及丙二醛(MDA)的影响。结果表明,随着暴露时间的增加及各处理组浓度的升高,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-PX)活性明显降低,丙二醛(MDA)水平明显上升,从而说明阿特拉津和毒死蜱单一及联合暴露会对东北小鲵蝌蚪的抗氧化酶系活性产生影响,进而产生毒理效应。当暴露21 d后,通过恢复实验,发现恢复末期与暴露末期相比,低浓度组和中浓度组上述3种酶活性变化不显著,而高浓度组上述3酶活性均有明显改善。

Effect of Atrazine and Chlorpyrifos on Oxidative Stress of Salamander (Hynobius leechii) Tadpoles from Northeastern China

The present experiment analyzed the effect of atrazine and chlorpyrifos single or joint exposure on the activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-PX) and malondialdehyde (MDA) at different time periods and concentrations in Salamander (Hynobius leechii) tadpoles from northeastern China. With the increase of exposure time and elevation of concentration in each treatment group, the activities of SOD, CAT and GSH-PX decreased significantly, while the level of MDA increased significantly (shown in Fig. 1, 3, 5), indicating that exposure of H. leechii tadpoles to atrazine and chlorpyrifos, single or joint, impairs antioxidant system and produces a toxic effect. After 21 days of exposure, the activities of various enzymes in the low or moderate concentration group was not different as compared to their counterparts at the end of revovery, while the high concentration group showed significant improvement of enzyme activities after recovery (shown in Fig. 2, 4, 6).