A preliminary study: the anti-proliferation effect of salidroside on different human cancer cell lines |
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Authors: | Xiaolan Hu Shuxin Lin Daihua Yu Shuifeng Qiu Xianqi Zhang Ruhuan Mei |
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Institution: | (1) Department of Pathology and Pathophysiology, Zhejiang University School of Medicine, 388Yuhangtang Road, Hangzhou, 310058, Zhejiang, China;(2) Department of Pathophysiology, Fourth Military Medical University, Xi’an, China |
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Abstract: | Salidroside (p-hydroxyphenethyl-beta-d-glucoside), which is present in all species of the genus Rhodiola, has been reported to have a broad spectrum of pharmacological properties. The present study, for the first time, focused
on evaluating the effects of the purified salidroside on the proliferation of various human cancer cell lines derived from
different tissues, and further investigating its possible molecular mechanisms. Cell viability assay and 3H] thymidine incorporation were used to evaluate the cytotoxic effects of salidroside on cancer cell lines, and flow cytometry
analyzed the change of cell cycle distribution induced by salidroside. Western immunoblotting further studied the expression
changes of cyclins (cyclin D1 and cyclin B1), cyclin-dependent kinases (CDK4 and Cdc2), and cyclin-dependent kinase inhibitors
(p21Cip1 and p27Kip1). The results showed that salidroside inhibited the growth of various human cancer cell lines in concentration- and time-dependent
manners, and the sensitivity to salidroside was different in those cancer cell lines. Salidroside could cause G1-phase or
G2-phase arrest in different cancer cell lines, meanwhile, salidroside resulted in a decrease of CDK4, cyclin D1, cyclin B1
and Cdc2, and upregulated the levels of p27Kip1 and p21Cip1. Taken together, salidroside could inhibit the growth of cancer cells by modulating CDK4-cyclin D1 pathway for G1-phase arrest
and/or modulating the Cdc2-cyclin B1 pathway for G2-phase arrest. |
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