拟南芥WUSCHEL基因在转基因烟草中的超表达(英文)

The Arabidopsis WUSCIHIEL (WUS） gene plays a key role in the specification of the stem cellsin the shoot apical meristem (SAM). A cDNA of WUShas been amplified with the RT-PCR approach fromArabidopsis. The plant overexpression vector was constructed. It was driven by a dual enhanced CaMV35Spromoter. The construct was transformed into tobacco (Nicotiana tabacum L.) via Agrobacterium mediation.Dramatic phenotypic changes appeared in the WUS overexpression transgenic plants. Aberrant celldivisions and ectopic organogenesis could be found in almost every aerial parts of the transgenic tobaccoexcept the meristems and the inner two floral whorls. The data showed a highly conserved function of WUSin tobacco, and suggested that WUS is involved in organogenesis. The leaves were malformed, whichstrongly matched those only described previously for plants grown in the presence of polar auxin transportinhibitors. It suggested a possible function of WUS in leaf development. These results provide usefulinformation for functional analysis of WUS and important biotechnological implication as well.

Analysis of Transgenic Tobacco with Overexpression of Arabidopsis WUSCHEL Gene

The Arabidopsis WUSCHEL {WUS) gene plays a key role in the specification of the stem cells in the shoot apical meristem (SAM). A cDNA of WUS has been amplified with the RT-PCR approach from Arabidopsis. The plant overexpression vector was constructed. It was driven by a dual enhanced CaMV35S promoter. The construct was transformed into tobacco (Nicotiana tabacum L) via Agrobacterium mediation. Dramatic phenotypic changes appeared in the WUS overexpression transgenic plants. Aberrant cell divisions and ectopic organogenesis could be found in almost every aerial parts of the transgenic tobacco except the meristems and the inner two floral whorls. The data showed a highly conserved function of WUS in tobacco, and suggested that WUS is involved in organogenesis. The leaves were malformed, which strongly matched those only described previously for plants grown in the presence of polar auxin transport inhibitors. It suggested a possible function of WUS in leaf development. These results provide useful information for functional analysis of WUS and important biotechnological implication as well.