Acetonitrile-hydrolysing enzymes in Rhodococcus erythropolis A10 |
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| Authors: | Acharya A Desai AJ |
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| Institution: | (1) Department of Microbiology and Biotechnology Centre, Faculty of Science, M.S. University of Baroda, Baroda –, 390002, India |
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| Abstract: | Rhodococcus erythropolis A10 metabolizes acetonitrile by a two step process involving nitrile hydratase (NHase) and amidase. Both the enzymes were inducible and low basal levels of activities were observed in the cells grown in the absence of acetonitrile (AN). Cobalt and iron enhanced NHase, while amidase showed iron dependence. Presence of glucose or ammonium sulphate (AS) failed to affect acetonitrile utilization. |
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| Keywords: | Nitrile hydratase amidase acetonitrile utilization Rhodococcus erthropolis A10 |
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