Ultrastructure of the metanephridial system in Aeolosoma bengalense (Annelida)

Summary The excretory system of Aeolosoma bengalense has been examined by light and electron microscopy. The system consists of seven serially arranged paris of metanephridia and six pairs of podocytes (referring to the first zoid of an animal chain). The podocytes surround blood spaces of the alimentary canal forming dorsoventrally running loops that emerge on both sides of it. The two elements of the system have a correlative position, each podocyte extending in close proximity to the funnel of a metanephridium. Only in the region of the first metanephridia are podocytes lacking. The nephrostome of the metanephridia consists of two cells, an inner one, the terminal duct cell, and an outer one enwrapping it, called the mantle cell. Nephrostomal cilia that extend into the coelomic space arise exclusively from the rim of the mantle cell whereas those of the terminal duct cell arranged on its luminal surface protrude into the canal forming a flame. The nephridial canal is ciliated throughout and is either intra- or extracellular. Its initial loops aggregate to form a compact organ, the nephridial body. The middle part of the duct constitutes a loop that ascends at each side of the alimentary canal where it is in intimate contact with its blood spaces. Ultrastructural features of the duct cells suggest a reabsorptive function in two regions, the nephridial body and the uppermost part of the loop. The terminal part of the duct passes through the nephridial body and opens ventrolaterally. Generally, the transverse vascular loops at the gut consist of one podocyte each. In the oesophageal region, where only one pair of podocytes is present, the loops connect the dorsal with the ventral longitudinal vessel. Three pairs of podocytes are present in the dilated region of the intestine emerging from its lateral wall and joining the median ventral vessel or blood spaces near by. In the hind gut, where two pairs of podocytes occur, the loops arise from the dorsolateral part and enter directly the ventral vessel. Cytological features of podocytes resemble those of other animals. The results are discussed on the basis of current theories on the function and the phylogenetic significance of excretory systems in the Annelida.Abbreviations bl basal lamina - bs blood space - bv blood vessel - cf ciliary flame - ci cilia - co connection of the vascular loop with the intestinal blood space - cu cuticle - db dense body - dc duct cell - di dictyosome - za zonula adhearens - dv dorsal vessel - ecb epicuticular body - ev endocytotic vesicle - ic intestinal cell - ici inner cilia - iv intestinal vessel - lm longitudinal muscle - mc mantle cell - mg midgut - mi mitochondrion - mv microvilli - nu nucleus - oci outer cilia - oe oesophagus - pc podocyte - pe pedicel - pel primary elongation of the podocyte - sm slit membrane - tc terminal duct cell - ve vesicle with heterogeneous contents - vv ventral vessel