Monolysocardiolipin: improved preparation with high yield |
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| Authors: | Kim Junhwan Hoppel Charles L |
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| Affiliation: | Department of Pharmacology, Case Western Reserve University, Cleveland, OH 44106, USA. |
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| Abstract: | A simple, high-yielding preparation of monolysocardiolipin (MLCL) by phospholipase A2 hydrolysis of cardiolipin (CL) in methanol on a semi-preparative scale is described. In methanol, phospholipase A2 preferentially hydrolyzes CL to MLCL. This selectivity results in ～80% yield of MLCL. The synthesized MLCL and dilysocardiolipin were characterized by NMR and ESI-MS/MS. Only the sn-2 position of CL was hydrolyzed by phospholipase A2 in methanol. |
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| Keywords: | cardiolipin phospholipase mass spectrometry dilysocardiolipin |
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