Microbial production of 2,3-butanediol from Jerusalem artichoke tubers by <Emphasis Type="Italic">Klebsiella pneumoniae</Emphasis> |
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Authors: | Li-Hui Sun Xu-Dong Wang Jian-Ying Dai Zhi-Long Xiu |
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Institution: | (1) Department of Bioscience and Biotechnology, School of Environmental and Biological Science and Technology, Dalian University of Technology, Linggong Road 2, 116024 Dalian, People’s Republic of China |
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Abstract: | 2,3-Butanediol is one of the promising bulk chemicals with wide applications. Its fermentative production has attracted great
interest due to the high end concentration. However, large-scale production of 2,3-butanediol requires low-cost substrate
and efficient fermentation process. In the present study, 2,3-butanediol production by Klebsiella pneumoniae from Jerusalem artichoke tubers was successfully performed, and various technologies, including separate hydrolysis and fermentation
(SHF) and simultaneous saccharification and fermentation (SSF), were investigated. The concentration of target products reached
81.59 and 91.63 g/l, respectively after 40 h in batch and fed-batch SSF processes. Comparing with fed-batch SHF, the fed-batch
SSF provided 30.3% higher concentration and 83.2% higher productivity of target products. The results showed that Jerusalem
artichoke tuber is a favorable substrate for 2,3-butanediol production, and the application of fed-batch SSF for its conversion
can result in a more cost-effective process. |
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Keywords: | 2 3-Butanediol Jerusalem artichoke Separate hydrolysis and fermentation Simultaneous saccharification and fermentation |
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