Tea leaf peroxidase--Isolation and partial purification

Tea leaf peroxidase occurred as the insoluble form in a homogenate.A large part of the peroxidase from tea leaves was solubilizedin a homogenate by adding Tween-80. After fractionation by 0.3saturation with (NH₄)₂SO₄ and filtration of the homogenate througha Sephadex G-25 column, specific activity of the peroxidasein the effluent increased about 25 times. Crude peroxidase wasfractionated into six components; A-l, A-2, B-l, B-2, B-3 andC, by DEAE- and CM-cellulose column chromatography. The fractionsshowed different mobilities on disc gel electrophoregram atpH 4.2. An isoelectric focusing experiment indicated that pivalues of the A-l and A-2 components are 5.4 and 5.0, repsectively. ¹ Present address: National Institute of Agricultural Science,Ministry of Agriculture and Forestry, Nishigahara, Kita-ku,Tokyo, Japan. (Received October 19, 1970; )