| 63例慢性乙肝e抗原阴性HBV基因多态性检测结果报告 |
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| 引用本文： | 许培仁,孙建华,朱艳荣,赵建龙,唐向荣. 63例慢性乙肝e抗原阴性HBV基因多态性检测结果报告[J]. 微生物学免疫学进展, 2006, 34(4): 52-53 |
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| 作者姓名： | 许培仁 孙建华 朱艳荣 赵建龙 唐向荣 |
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| 作者单位： | 1. 河南大学淮河医院检验科,开封,475000
2. 上海微系统与信息技术研究所,上海,200050 |
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| 摘 要： | 用地高辛标记引物酶显色法,检测了63例e抗原阴性慢性肝炎HBV基因多态性。结果突变率为53.9%(34/63)。前C/C区1896位突变率最高为49.2%(31/63),1814位38.1%(24/63);BCP区1762位、1764位均为39.7%(25/63),552位突变率为14.3%(9/63)。该检测方法灵敏度高,简便易行。严格控制杂交温度及显色温度是检测操作的关键。
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| 关 键 词： | 寡核苷酸芯片 乙型肝炎病毒 基因多态性 杂交 |
| 文章编号： | 1005-5673（2006）04-0052-02 |
| 收稿时间： | 2006-02-23 |
| 修稿时间： | 2006-07-06 |
Detection gene polymorphism of HBV in 63 cases of eAg(-) chronic HBV infections |
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| XU Pei-ren ,SUN Jian-hua, ZHU Yan-rong,et al.. Detection gene polymorphism of HBV in 63 cases of eAg(-) chronic HBV infections[J]. Progress In Microbiology and Immunology, 2006, 34(4): 52-53 |
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| Authors: | XU Pei-ren SUN Jian-hua ZHU Yan-rong et al. |
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| Affiliation: | 1 Huai He hosiptal of Henan university, Kaifeng 475000 ,China;2 Shanghai Insititute of Microsystern and Information Techology Academy of Sciences, Shanghai 200050, China |
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| Abstract: | Detection of HBV gene polymorphism was carried out by using of enzyme coloration with digoxin-labeled primers in 63 cases of eAg(-) chronic HBV infections.And the detected mutation rate was 53.9%(34/63).The mutation rate in 1896th position of pre-c/c region was the highest as 49.2%(31/63);in 1814th was 38.1%(24/63);the mutation rate in 1762th and 1764th in BCP region were the same as 39.7%(25/63).The mutation rate in 552th position in P region was 14.3%(9/63).The method is more sensitive and easily operated in clinical practice.The key points are strictly control of temperature both in the hybridization and colour development in the detection. |
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| Keywords: | Oligonucleotide array Hepatitis B virus(HBV) Gene polymorphism Hybridization |
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