Hydrogen Peroxide-Dependent Oxidation of 3,4-Dihydroxyphenylalanine in Vacuoles of Mesophyll Cells of Vicia faba L. Participation of Peroxidase in the Oxidation |
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Authors: | Takahama Umeo; Egashira Takeshi |
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Institution: | Department of Biology, Kyushu Dental College Kitakyushu, 803 Japan |
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Abstract: | Peroxidase activity and 3,4-dihydroxyphenylalanine (DOPA) werefound in vacuoles isolated from mesophyll protoplasts of Viciafaba L. A peroxidase isozyme localized in vacuoles migratedto the cathode during electrophoresis at pH 8.7, indicatingthat the vacuole peroxidase was a basic isozyme. When isolatedvacuoles were treated with 2 mM H2O2, dopachrome, a productof oxidation of DOPA, was formed in a reaction that was inhibitedby KCN and NaN3. These results suggest that DOPA can serve asa donor of electrons to the peroxidase in vacuoles. (Received December 25, 1989; Accepted March 22, 1990) |
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