Oxidation of carbazole to 3-hydroxycarbazole by naphthalene 1,2-dioxygenase and biphenyl 2,3-dioxygenase |
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| Authors: | Sol M Resnick Daniel S Torok David T Gibson |
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| Institution: | The Department of Microbiology and Center for Biocatalysis, College of Medicine, The University of Iowa, Iowa City, IA 52242, USA |
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| Abstract: | Abstract Naphthalene 1,2-dioxygenase from Pseudomonas sp. NCIB 9816-4 and biphenyl dioxygenase from Beijerinckia sp. B8/36 oxidized the aromatic N-heterocycle carbazole to 3-hydroxycarbazole. Toluene dioxygenase from Pseudomonas putida F39/D did not oxidize carbazole. Transformations were carried out by mutant strains which oxidize naphthalene and biphenyl to cis -dihydrodiols, and with a recombinant E. coli strain expressing the structural genes of naphthalene 1,2-dioxygenase from Pseudomonas sp. NCIB 9816-4. 3-Hydroxycarbazole is presumed to result from the dehydration of an unstable cis -dihydrodiol. |
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| Keywords: | Carbazole Biotransformation Naphthalene dioxygenase Biphenyl dioxygenase Pseudomonas |
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