镍对高冰草幼苗生长及活性氧代谢的影响

本文对镍处理下高冰草幼苗生长,茎部和根部丙二醛(MDA)含量,可溶性蛋白含量,超氧化物岐化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性及叶片光合色素含量的变化进行了研究.结果表明,低浓度镍(10 μmol·L~(-1))对高冰草幼苗生长无显著抑制效应,但随镍浓度增加各项生长指标显著减少,且镍对高冰草幼苗根部损伤更为显著.光合色素含量随镍浓度升高呈减少趋势.高冰草茎部和根部MDA含量与镍浓度呈显著正相关关系.根部可溶性蛋白含量随镍浓度升高呈增加趋势.低浓度镍处理(10 μmol·L~(-1))下,SOD、POD和CAT活性均有所提高,保护酶系统处于正常状态,但随镍浓度升高,3种保护酶活性受到不同程度抑制,保护酶系统平衡被破坏.

Abstract：

Agropyron elongatum seedlings were exposed to different concentration Ni to study their growth and the changes in MDA and soluble protein contents and SOD, POD and CAT ac-tivities in their shoots and roots, and the photosynthetic pigment content in their leaves. Low con-centration (10 μmol·L~(-1)) Ni had no significant inhibitory effects on the seedlings growth, but the growth indices decreased significantly with increasing Ni concentration, being more markedly for roots than for shoots. The leaf photosynthetic pigment content had a decreasing trend with in-creasing Ni concentration, while the MDA content in shoots and roots had significant positive cor-relation with Ni concentration. The soluble protein content in roots increased with increasing Ni concentration. Under low concentration (10μmol·L~(-1)) Ni, the SOD, POD and CAT activities all increased, and the protective enzyme system was well-balanced. When the Ni concentration was increasing, the three enzyme activities were inhibited to different extents, and the balance of protective enzyme system was broken.

Effects of Ni stress on Agropyron elongatum seedlings growth and reactive oxygen species metabolism

Agropyron elongatum seedlings were exposed to different concentration Ni to study their growth and the changes in MDA and soluble protein contents and SOD, POD and CAT ac-tivities in their shoots and roots, and the photosynthetic pigment content in their leaves. Low con-centration (10 μmol·L~(-1)) Ni had no significant inhibitory effects on the seedlings growth, but the growth indices decreased significantly with increasing Ni concentration, being more markedly for roots than for shoots. The leaf photosynthetic pigment content had a decreasing trend with in-creasing Ni concentration, while the MDA content in shoots and roots had significant positive cor-relation with Ni concentration. The soluble protein content in roots increased with increasing Ni concentration. Under low concentration (10μmol·L~(-1)) Ni, the SOD, POD and CAT activities all increased, and the protective enzyme system was well-balanced. When the Ni concentration was increasing, the three enzyme activities were inhibited to different extents, and the balance of protective enzyme system was broken.