Characterization of Albumins fromLupinus luteus L. Cotyledons

The cotyledons of yellow lupin seeds contain 15.4 mg of the albumins, water soluble proteins, per 100 mg of dry matter. These proteins were resolved by SDS gel electrophoresis into 13 polypeptides with relative molecular mass, Mr, between 16 000 and 84 000. Isoelectrofocusing analyses of the albumins showed that they were composed of six components with isoelectric points between 4.1 and 5.5. These results have indicated that the albumins are acid proteins and exhibited molecular and charge heterogeneity.