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GTPase from rod outer segments: characterization by photoaffinity labeling and tryptic peptide mapping |
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| Authors: | D J Takemoto B E Haley J Hansen O Pinkett L J Takemoto |
| Institution: | 2. the Department of Biochemistry, the University of Wyoming, Laramie, Wyoming 82071 USA |
| Abstract: | The photoaffinity label γ-32P]8-N3GTP has been used to identify GTP-binding components in highly purified preparations of GTPase from bovine rod outer segments. These preparations contain two major polypeptides of 37,000 and 39,000 daltons. In the presence of photolyzing radiation, γ-32P]8-N3GTP is covalently attached to the 37,000 dalton polypeptide. Tryptic peptide mapping of this polypeptide indicates that it is highly related to the 39,000 dalton species that has been previously identified as a GTP-binding component. |
| Keywords: | ROS rod outer segments Buffer B 10 mM 4-morpholinepropanesulfonic acid 0 1 mM phenylmethylsulfonyl fluoride 1 mM dithiothreitol pH 7 5 DPCC diphenyl carbamyl chloride |
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