红豆杉种质资源遗传多样性的AFLP分析

目的：通过对5份红豆杉种质资源的AFLP分析,探求各种质间的遗传多样性。方法：采用扩增片段长度多态性（AFLP）标记,在DNA水平上进行遗传多样性研究,筛选了32对选择性扩增引物,将扩增出的条带作为原始矩阵,用NTSYS-PC软件计算并分析了红豆杉种质间的相似度,构建了遗传系统进化树。结果：（1）SDS法提取的红豆杉基因组DNA质量较佳,能够满足AFLP分析的要求;（2）从32对选择性扩增引物中,筛选出10对多态性较强、带型较好、分辨率较高的组合;（3）构建了红豆杉AFLP指纹图谱,将5个红豆杉种质全部区分开来;（4）通过构建进化树,把5个种质分成3类。结论：红豆杉种质资源有丰富的遗传多样性。

AFLP Analysis of Genetic Diversity of Taxus chinensis var.mairei

Objectlve：The AFLP analysis of 5 accessions of Taxus was conducted to explore genetic diversities among germplasm. Methods： Genetic diversities at DNA level among the germplasm of Leonurus heterophyllus were studied by the Amplified Fragment Length Mprphologies （AFLP） marker, and 32 pairs of selective amplification primer were identified. The similarities among the germplasm of Leonurus heterophyllus were calculated and analyzed by the soft of NTSYS - PC, and genetic evolution tree was developed, using amplified fragments as primary matrix. Results： （ 1 ） The quality of genomic DNA of Taxus extracted by SDS method was good enough to meet the requirement of AFLP analysis. （2） Ten combinations with multiple morphologies, clear bands and high discrimination rate were identified from 32 pairs of selective amplification primers. （3）The AFLP fingerprinting was developed, which can differentiate each of 5 accessions of Taxus. （4）Five accessions of Taxus could be classified into three types based on the evolution tree. Conclusion： There were richer genetic diversities among the germplasm of Taxus, and certain relationships were found between some of morphological characteristics and genotvoes.