A novel simple satellite DNA is colocalized with the Stalker retrotransposon in Drosophila melanogaster heterochromatin |
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Authors: | Makunin I V Pokholkova G V Kholodilov N G Zakharkin S O Bonaccorsi S Dimitri P Zhimulev I F |
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Institution: | (1) Institute of Cytology and Genetics Novosibirsk, 630090, Russia, RU;(2) Dipartimento di Genetica e Biologia Molecolare Universita di Roma “La Sapienza”, Piazzale A. Moro 5 00185 Rome, Italy e-mail: dimitri@axcasp.caspur.it Tel.: +39-6-49912655; Fax: +39-6-4456866, IT |
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Abstract: | In the T(1;2)dor
var7
multibreak rearrangement the distal 1A-2B segment of the X chromosome of Drosophila melanogaster is juxtaposed to an inverted portion of the heterochromatin of chromosome 2. Analysis of mitotic chromosomes by a series of banding techniques has permitted us precisely to locate the heterochromatic
breakpoint of this translocation in the h42 region of 2R. Cloning and sequencing of the eu-heterochromatic junction revealed that the translocated 1A-2B fragment is joined to (AACAC)n repeats, which represent a previously undescribed satellite DNA in D. melanogaster. These repeated sequences have been estimated to account for about 1 Mb of the D. melanogaster genome. The repeats are located mainly in the Y chromosome and in the heterochromatin of the right arm of chromosome 2 (2Rh), where they are colocalized with the Stalker retrotransposon.
Received: 3 October 1998 / Accepted: 3 December 1998 |
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Keywords: | Drosophila melanogaster Constitutive heterochromatin Satellite DNA Transposable elements |
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