High expression of osteoglycin decreases the metastatic capability of mouse hepatocarcinoma Hca-F cells to lymph nodes |
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Authors: | Cui Xiaonan Song Bo Hou Li Wei Zhiyi Tang Jianwu |
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Institution: | Department of Oncology, the First Affiliate Hospital of Dalian Medical University, Dalian 116011, China;Department of narcotherapy, Dalian Medical University, Dalian 116044, China;Department of Anesthesiology, The Second Affiliate Hospital of Dalian Medical University, Dalian 116027, China |
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Abstract: | Osteoglycin, one of the matrix molecules, belongs to the small leucine-rich proteoglycan gene family and might play important roles in cell growth and differentiation and in pathological processes such as fibrosis and cancer growth. In this study, a eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into mouse hepatocarcinoma Hca-F cells to evaluate the contribution of osteoglycin to the malignant behavior of Hca-F. It was found that Hca-F cells transfected with pIRESpuro3 osteoglycin (+) showed significantly decreased potential for both migration and invasion. Furthermore, Hca-F cells transfected with osteoglycin showed decreased metastatic potential to peripheral lymph nodes. However, proliferation potential and adhesive capacity of Hca-F cells to differentprotein substrates were not influenced by osteoglycin transfection. In summary, high expression of osteoglycin decreases the metastatic capability of Hca-F to lymph nodes. |
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Keywords: | osteoglycin transfection hepatocellular carcinoma neoplasm metastasis |
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