| Affiliation: | (1) Faculty of Technology, Bulevar Cara Lazara 1, 21000 Novi Sad, Yugoslavia;(2) Faculty of Science, Bulevar Cara Lazara 1, 21000 Novi Sad, Yugoslavia;(3) Faculty of Agriculture, Bulevar Cara Lazara 1, 21000 Novi Sad, Yugoslavia |
| Abstract: | In the present communication, a general method for the kinetic analysis of random bisubstrate mechanisms is described. The method comprises a stepwise application of the following kinetic and ligand-binding experiments: determination of steady-state kinetic constants, product inhibition patterns, maximum rate relationships, application of alternate substrates, application of dead-end inhibitors, direct binding of substrates, kinetic isotope effects, and isotope exchange studies. This general method was applied to a practical example: a yeast alcohol dehydrogenase-catalyzed oxidation of 2-propanol by NAD+ at pH 7.0, 25°C. It was found that this fully reversible reaction proceeds by a steady-state random Bi-Bi mechanism, whereby both dead-end complexes are formed. |
