Degradation patterns and intermediates in the anaerobic digestion of glucose: Experiments with 14C-labeled substrates |
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| Authors: | A. Cohen A. van Deursen J. G. van Andel A. M. Breure |
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| Affiliation: | (1) Laboratory for Microbiology, University of Amsterdam, Nieuwe Achtergracht 127, 1018 WS Amsterdam, The Netherlands;(2) Laboratory for Chemical Technology, University of Amsterdam, Nieuwe Achtergracht 127, 1018 WS Amsterdam, The Netherlands;(3) Present address: CSM Suiker b.v., P.O. Box 349, 1000 AH Amsterdam, The Netherlands |
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| Abstract: | A mineral salts medium containing 1% (w/v) glucose was subjected to anaerobic digestion in an upflow reactor. Performance with respect to utilization of glucose was monitored by collection of fermentation gases and calculation of carbon mass balances. Sub-samples of bacterial supennsions from the upflow reactor were incubated with (U-14C)-glucose, (U-14C)-acetate, (2-14C)-propionate, (1-14C)-butyrate or 14C-carbonate. Individual radioactive products in samples from incubation mixtures were analysed by radio gas chromatography.Quantitatively, acetate and propionate were the only important intermediates in glucose degradation by glucose-adapted sludge, with acetate accounting for the largest part of intermediary fatty acid flux.Abbreviations used ATP Adenosine Triphosphate - TIC Total Inorganic Carbon - TOC Total Organic Carbon - VFA Volatile Fatty AcidsThe research described in this publication was supported by the Dutch Ministry of Public Health and Environmental Protection. |
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