| 树干毕赤酵母NLP31发酵产乙醇的研究 |
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| 引用本文： | 朱均均,勇强,徐勇,陈尚钘,余世袁.树干毕赤酵母NLP31发酵产乙醇的研究[J].生物加工过程,2010,8(1):1-5. |
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| 作者姓名： | 朱均均 勇强 徐勇 陈尚钘 余世袁 |
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| 作者单位： | 1. 南京林业大学,林木遗传与生物技术省部共建教育部重点实验室,南京,210037
2. 江西农业大学,林学院,南昌,330045 |
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| 基金项目： | 国家自然科学基金资助项目，国家高技术研究发展计划(863计划)资助项目，江苏省高校自然科学重大基础研究项目 |
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| 摘 要： | 研究了树干毕赤酵母NLP31在木糖质量浓度为45 g/L的3种发酵培养基Ⅰ、Ⅱ和Ⅲ上发酵3轮的发酵性能以及在45 g/L木糖或混合糖(葡萄糖30 g/L,木糖15 g/L)的发酵培养基Ⅲ上的代谢历程。结果表明:树干毕赤酵母NLP31在发酵培养基Ⅲ上,乙醇浓度和乙醇得率均达到最高,分别为(17.29±0.15)g/L和(84.65±0.58)%。在45 g/L木糖或混合糖(葡萄糖30 g/L,木糖15 g/L)的发酵培养基Ⅲ上的代谢历程表明:混合糖发酵达到最大乙醇得率的时间仅为12 h,要比单一木糖发酵缩短了8 h。树干毕赤酵母NLP31在以廉价的无机N源为发酵培养基上的乙醇发酵性能高,能够降低燃料乙醇的生产成本。
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| 关 键 词： | 乙醇 发酵 戊糖 树干毕赤酵母 |
Fermentation production of ethanol by Pichia stipitis NLP31 |
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| ZHU Jun-jun,YONG Qiang,XU Yong,CHEN Shang-xing,YU Shi-yuan.Fermentation production of ethanol by Pichia stipitis NLP31[J].Chinese Journal of Bioprocess Engineering,2010,8(1):1-5. |
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| Authors: | ZHU Jun-jun YONG Qiang XU Yong CHEN Shang-xing YU Shi-yuan |
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| Institution: | ZHU Jun-jun1,YONG Qiang1,XU Yong1,CHEN Shang-xing2,YU Shi-yuan1(1 Key Laboratory of Forest Genetics , Biotechnology of the Ministry of Education,Nanjing Forestry University,Nanjing 210037,China,2 College of Forestry,Jiangxi Agricultural University,Nanchang 330045,China) |
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| Abstract: | Fermentation of Pichia stipitis NLP31 on three types of media composed of sugars of 45 g/L xylose or mixture sugars (glucose 30 g/L, xylose 15 g/L) in 3 batches were investigated. The results showed that the ethanol concentration were the highest values by (17.29±0.15) g/L and yield of (84.65±0.58)% on fermentation medium III. The production course showed that the fermentation time of mixture sugar reached its highest ethanol level by 12 h, which was 8 h less than the control time with xylose alone. The fermentability of Pichia stipitis NLP31 was hight on the fermentation medium with less expensive inorganic nitrogen source, thus reducing the cost of the fuel ethanol production. |
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| Keywords: | ethanol fermentation pentose Pichia stipitis |
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