Lysine-requiring mutants ofAspergillus ochraceus |
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| Authors: | D. Saha T. K. Das |
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| Affiliation: | (1) Department of Biochemistry and Biophysics, University of Kalyani, Kalyani, 741 235 Nadia, West Bengal, India |
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| Abstract: | Seven mutants ofAspergillus ochraceus unable to produce lysine have been selected by treating conidia of the wild type with N-methyl-N′-nitro-N-nitrosoguanidine (MNNG), at pH 6.4. Complementation analysis revealed that MNNG had caused a mutation at a single locus. Growth studies indicated the growth requirement for lysine in the mutants. Lysine-requiring mutants were further characterized by measurement of colony extension rate at various lysine concentrations. |
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