Mating,male Ixodes scapularis express several genes including those with sequence similarity to immunoglobulin-binding proteins and metalloproteases |
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Authors: | Packila Mark Guilfoile Patrick G |
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Institution: | (1) Biology Department, Bemidji State University, 1500 Birchmont Dr., NE, Bemidji, MN 56601, USA;(2) Present address: Northwoods DNA, Inc, 44526 Country Road 3, Becida, MN 56678, USA |
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Abstract: | We created a cDNA library from mating, male Ixodes scapularis ticks and screened the library with a subtracted probe to eliminate genes common to feeding female and mating male I. scapularis ticks. A total of seven unique cDNAs were identified in this screen. One cDNA had sequence similarity to the IGBP-MC gene
from Rhipicephalus appendiculatus, and another cDNA potentially encodes a protein with similarity to metalloproteases. RT-PCR, using RNA isolated from male
and female I. scapularis ticks, confirmed that these genes are expressed in male, but not female ticks. The remaining five cDNAs did not match any
sequences in the GenBank database.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | tick mating immunoglobulin binding protein metalloprotease Ixodes scapularis |
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