Structural mannoproteins released by β-elimination from Candida albicans cell walls |
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Authors: | S Mormeneo A Marcilla M Iranzo R Sentandreu |
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Institution: | Departament de Microbiología, Facultat de Farmàcia, Universitat de València, Avgda. Vicent Andres Estelles s/n, 46100 Burjassot, Valencia, Spain; Institut d'Investigacions Citológiques, FundacióValenciana d'Investigacions Biomédiques2, València, Spain |
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Abstract: | Abstract Mild alkaline solutions (β-elimination), after removing the non-covalently bonded wall materials by hot SDS, released 13% and 26% of remaining wall proteins from mycelial and yeast cells of Candida albicans , respectively. When the β-elimination was carried out after digestion of the walls with chitinase, four-fold more proteinaceous materials were released from mycelium and a similar amount in yeast walls. The solubilized materials were shown to be highly polydisperse, and endo-glycosidase H reduced their polydispersity and molecular masses, revealing different electrophoretic patterns in yeast and mycelial cell walls. The solubilized mycelial proteins carried N-glycosidic sugar chains and the epitopes recognized by two monoclonal antibodies were preserved, although showing a different behaviour in yeast walls. These results are consistent with the idea that significant amounts of intrinsic O-glycosylated mannoproteins are interconnected in the walls of C. albicans . |
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Keywords: | Mannoproteins Cell wall β-Elimination Candida albicans |
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