Isolation and characterization of Methanocorpusculum parvum,gen. nov., spec. nov., a new tungsten requiring,coccoid methanogen |
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Authors: | G. Zellner C. Alten E. Stackebrandt E. Conway de Macario J. Winter |
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Affiliation: | (1) Lehrstuhl für Mikrobiologie, Universität Regensburg, D-8400 Regensburg, Germany;(2) Institut für Allgemeine Mikrobiologie, Christian-Albrechts-Universität, D-2300 Kiel, Germany;(3) Wadsworth Center for Laboratories and Research, New York State Department of Health, 12201 Albany, NY, USA |
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Abstract: | A new mesophilic, monotrichously flagellated methane-producing coccus of 1m in diameter was isolated from an anaerobic sour whey digester, originally inoculated with sewage sludge. Growth and methane production were observed with H2/CO2, formate and — less effectively — with 2-propanol/CO2. The isolate grew at temperatures between 15° C and 45° C with the optimum at around 37° C. Acetate, yeast extract and tungstate were required in the medium. Clarified rumen fluid stimulated growth.The DNA of the new methanogen has a G+C content of 48.5 mol%. Comparative 16 S rRNA oligonucleotide cataloguing allows to define the new isolate as a member of a new genus of the order Methanomicrobiales. Further evidence for this is provided by the antigenic crossreactivity with anti-S probes and by metabolic features.Because of its small size the new methanogen is named Methanocorpusculum parvum.This work was supported by a grant of the Deutsche Forschungsgemeinschaft DFG to J. W. and E. S. Immunologic studies were supported in part by grants No. DE-FGO2-84 R 13197 from the U.S. Department of Energy, and No. 261.81/82 from the North Atlantic Treaty Organization (NATO) |
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Keywords: | Archaebacteria Methanogens Methanocorpusculum parvum Taxonomy 16 S rRNA Serological probes Physiology Tungsten Cofactors Cytochrome |
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