Phylogenetic analysis of the internal transcribed spacer region of Japanese Lilium species |
| |
Authors: | J G Dubouzet K Shinoda |
| |
Institution: | (1) Hokkaido National Agricultural Experiment Station, Hitsujigaoka 1, Toyohira-ku, Sapporo 062 Japan Tel.: 11-857-9304+81 Fax: 11-859-2178+81 E-mail: joseph@cryo.affrc.go.jp, JP |
| |
Abstract: | The DNA from 16 Lilium species and one variety endemic to or naturalized in Japan were obtained and their internal transcribed spacer regions of
nuclear ribosomal DNA (nrDNA) were amplified by PCR and sequenced by cycle sequencing. Phylogenetic analysis of the ITS sequences
supported the validity of Comber’s classification system. It has also provided molecular evidence for the transfer of Lilium dauricum to sect. Sinomartagon. The phylogenetic relationships revealed by ITS DNA analysis were supported by previously published crossability data. The
molecular phylogeny of Japanese Lilium species was discussed with reference to the putative migration routes of these species.
Received: 30 June 1998 / Accepted: 19 October 1998 |
| |
Keywords: | Lilium Molecular systematics ITS DNA sequences Phylogeny |
本文献已被 SpringerLink 等数据库收录! |