Zinc-binding proteins detected by protein blotting |
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Authors: | A Mazen G Gradwohl G de Murcia |
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Institution: | Institut de Biologie Moléculaire et Cellulaire du CNRS, Strasbourg, France. |
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Abstract: | The Western blotting technique was used for the detection of zinc-binding proteins. Proteins were separated electrophoretically on 15% polyacrylamide-sodium dodecyl sulfate minigels, the gels were soaked in a reduction buffer, and the proteins were transferred to nitrocellulose filters. Zinc-binding proteins were probed with radioactive zinc (65Zn) and were detected by autoradiography. This technique allows the detection of as little as 20 to 100 pmol of zinc metalloproteins. |
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