Immunoreactive dynorphin-A in bovine anterior pituitary and its in vitro release |
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| Authors: | C C Chao P V Malven |
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| Affiliation: | 1. United States Environmental Protection Agency, National Health & Environmental Effects, Research Laboratory, Gulf Ecology Division, 1 Sabine Island Drive, Gulf Breeze, FL 32561, USA;2. United States Environmental Protection Agency, Region 2, 290 Broadway, New York, NY 10007, USA;1. McArdle Laboratory for Cancer Research, University of Wisconsin-Madison, Madison, WI;2. Institute of Immunopharmaceutical Sciences, School of Pharmaceutical Sciences, Shandong University, Shandong Province, China;3. INSERM U1287, Gustave Roussy Cancer Center, Villejuif, France;4. Department of Medicine, University of Wisconsin School of Medicine and Public Health, University of Wisconsin Carbone Cancer Center, Madison, WI;5. Chemical Biology and Molecular Medicine Program, Moffitt Cancer Center, Tampa, FL;6. Medical School, Sigmund Freud University, Vienna, Austria;7. Department of Hematology, Gustave Roussy Cancer Center, Villejuif, France;8. Université Paris-Saclay, Faculté de Médecine, Le Kremlin-Bicêtre, France;9. Division of Hematology, Department of Internal Medicine, Mayo Clinic, Rochester, MN |
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| Abstract: | The anterior lobe (AL) of the bovine pituitary contained and released, during in vitro culture, a form of immunoreactive dynorphin-A (ir-DYN-A) larger than that occurring in neural tissue. Bovine AL tissue from intact females contained less ir-DYN-A than did AL tissue from castrated males. Enzymatically dispersed AL cells contained and released ir-DYN-A in vitro. Preincubation of dispersed AL cells for 18 hr, rather than 1.5 hr, increased the content and release of ir-DYN-A as well as LH. Addition of gonadotropin-releasing hormone (GnRH) to tissue slices or dispersed cells stimulated release of LH, but in contrast to published observations from rat AL, GnRH had no effect on release of ir-DYN-A. Addition of estradiol-17 beta, with or without progesterone, increased release of ir-DYN-A but not LH during 2-hr cultures. In summary, bovine AL contains and releases in vitro a large molecular weight form of ir-DYN-A. Although this ir-DYN-A was not coreleased with LH, a reproductive role was suggested by in vivo and in vitro effects of gonadal hormones on ir-DYN-A in the bovine anterior pituitary. |
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