Properties of a basement membrane-related glycoprotein synthesized in culture by a mouse embryonal carcinoma-derived cell line. |
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Authors: | A E Chung R Jaffe I L Freeman J P Vergnes J E Braginski B Carlin |
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Affiliation: | 1. Department of Biological Sciences University of Pittsburgh Pittsburgh, Pennsylvania 15260 USA;22. Department of Pathology University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania 15261 USA;3. Department of Ophthalmology University of Pittsburgh School of Medicine Pittsburgh, Pennsylvania 15261 USA |
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Abstract: | Two glycoproteins, GP-1 and GP-2, have been isolated from an extracellular membrane synthesized in cell culture by an embryonal carcinoma-derived cell line. The amino acid and carbohydrate compositions have been determined. Both proteins are rich in half-cystine residues and contain approximately 12-15% carbohydrate. Antibodies have been obtained against one of the glycoproteins, GP-2, in rabbits. The antibody reacts with basement membranes from adult mouse and human kidney glomeruli and tubules, and all basement membranes tested from mouse embryonic tissues. The molecular properties of GP-2 are superficially similar to LETS protein; however, immunological and other criteria show that they are distinct proteins. The presence of LETS protein and GP-2 in basement membranes suggests that there are subtle interactions which are important in adhesion of epithelial cells to basement membranes. |
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