Isolation of mutants with improved production of cAMP from Microbacterium sp. no. 205 (ATCC21376)

Summary Mutants were isolated from Microbacterium sp. no. 205 (ATCC 21376) producing 13–30mm cyclic adenosine 3,5-monophosphate (cAMP) by salvage biosynthesis, through sequential improvements of the bacterium for the purpose of improving cAMP production. The mutants produced 50–75 mm cAMP on 100 mm inosine 5-monophosphate as a precursor. Mutants resistant to the inhibition of growth by cAMP at high concentrations were isolated; the resistance was one of four characteristics effective for improved production of cAMP.