PHOTOCHEMICAL ACTIVITIES OF SONICATED LAMELLAE OF SPINACH CHLOROPLASTS WITH SPECIAL REFERENCE TO THE ACTION OF CRS (CYTOCHROME C REDUCING SUBSTANCE) |
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Authors: | FUJITA, YOSHIHIKO MURANO, FUMIO |
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Affiliation: | Institute of Applied Microbiology, University of Tokyo Tokyo |
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Abstract: | The photooxidation of reduced DPIP with NADP and O2 as finalelectron acceptors was studied with sonicated lamellae of spinachchloroplasts, with special reference to the possible role ofCRS, a newly discovered factor of photochemical system I. Acorrelation between inactivation of NADP-photoreduction anddissolution of CRS was observed on treatment of the lamellaewith various organic solvents. The treatment also suppressedthe O2-linked photooxidation of reduced DPIP, although the suppressionwas not so marked as in the former reaction. The suppressedphotooxidation of reduced DPIP (with O2 and NADP as electronacceptors) was partially restored on addition of dyes of negativeredox potential, such as methyl viologen. The experimental resultsindicate that a factor participates in the reactions of photochemicalsystem I, probably functioning as an electron carrier couplingthe photooxidation of P700 with the reduction of ferredoxinor O2. The finding that the above mentioned activity of thefactor is destroyed by treatments which are effective in solubilizingCRS from the chloroplasts suggests the identity of the factorwith CRS. 1 Present address: Nomura Research Institute of Technology andEconomics, Kamakura, Kanagawa. |
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