以人工合成多肽为抗原的戊型肝炎病毒抗体检测方法的建立和评价

酶联法是ＨＥＶ感染的主要检测方法，多采用基因表达产物为抗原。近来由于人工合成多肽抗原克服了基因重组抗原制备复杂、非特异结构多、难以纯化的缺点，已广泛用于多种病毒感染的检测。根据已发表的ＨＥＶ氨基酸序列的保守性及亲水性等特点，设计合成了两个多肽ＥＳＰ１和ＥＳＰ２，分别位于ＯＲＦ２和ＯＲＦ３区，以此为混合抗原建立了检测ＨＥＶ抗体的间接ＥＬＬＳＡ法，与市售优质试剂比较，其阳性符合率为９７．７５％，阴性符合率为９９．４３％，总符合率为９８．８６％。该方法灵敏度高、特异性强、安全快速，是检测ＨＥＶ感染和流行病学调查的理想方法。

The Establishment and Evaluation of a Method for Detecting Anti-HEV Using Peptides

ostly employing recombinant antigen,indirect enzyme linked immunosorbent assay is usedto detect HEV infection.Since synthetic peptides can be synthetised easily and have less nonspe-cific structures,they have been extensively used in the laboratory diagnosis of viral diseases.Basedon the reported nucleotide sequence of HEV genome and conservatism and hydrophilicity of aminoacid sequence,two synthetic peptides from ORF2 and ORF3 were prepared and used to developean EIA for detecting Anti-HEV,Detected 264 clinical specimens,the coincident rate for positivewas 97.75 % and for negative 99.43%,as comparing with DBL reagent(Singapore).Thismethod has a high sensitivity,specificity safety and speed.It is an ideal method for detection ofHEV infection and survey of HEV epidemiology.