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Rapid fluorometric test for the quantitative determination of deoxynivalenol in raw cereals
Authors:M Hafner  Z Kubus  M Freudenschuss  E M Binder  R Krska
Institution:(1) Christian Doppler Laboratory for Mycotoxin Research Department of Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences, Konrad Lorenz Stra\e 20, 3430 Vienna, Tulln, Austria;(2) Biopure Referenzsubstanzen GmbH, Technopark 1, 3430, Tulln, Austria;(3) Romer Labs Diagnostic GmbH, Technopark 1, 3430, Tulln, Austria;(4) Konrad Lorenz Stra\e 20, 3430, Tulln, Austria
Abstract:Fast test systems fulfilling legislative specifications are required to determine mycotoxin contamination in unprocessed cerealse.g. at grain elevators, import and export terminals, or the milling and brewing industry to prevent contamination of food and feed. We describe the first tests of a novel fluorescence-based test for deoxynivalenol (DON), which will be commercially available within the next few months. The analytical procedure of the new test takes less than 15 minutes for extraction, purification, derivatization and measurement. The system’s advantage is its speed and easy procedure providing quantitative DON determination. To ensure an international standard, the validation procedure for wheat, barley and maize will be performed following USDA/GIPSA requirements (03/2006) for DON tests. Presented at the 28th Mykotoxin-Workshop, Bydgoszcz, Poland, May 29–31, 2006 Financial support: Christian Doppler Society and Government of Lower Austria
Keywords:deoxynivalenol  fluorescence test  rapid test kit  mycotoxin analysis
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