Free-Solution, Label-Free Protein-Protein Interactions Characterized by Dynamic Light Scattering |
| |
Authors: | Amy D. Hanlon Michael I. Larkin Ryan M. Reddick |
| |
Affiliation: | Department of Research and Development, Wyatt Technology, Santa Barbara, California |
| |
Abstract: | We report a free-solution, label-free method for quantitative characterization of macromolecular interactions using dynamic light scattering, a temperature controlled plate reader, and a multiwell concentration gradient. This nondestructive technique enabled determination of stoichiometry of binding, equilibrium dissociation constant, and thermodynamic parameters, as well as the impact of temperature, buffer salinity, and a small-molecule inhibitor. The low volume capability of dynamic light scattering reduced the required sample to 426 pmol/experiment, with detection limits for 150-kDa proteins anticipated to be in the low femtomole range. |
| |
Keywords: | |
本文献已被 ScienceDirect 等数据库收录! |
|