Kinetics of mycelial protoplast formation inGibberella fujikuroi andTrichoderma reesei

Mycelial protoplasts ofGibberella fujikuroi andTrichoderma reesei were prepared using Novozym 234 and linear relationship between the yield of the protoplasts and mycelial concentration up to 2 mg dry mass per mL was observed. The optima for concentration of the enzyme, the age of the culture and the contact time of the enzyme with mycelial cells were 2 g/L, 25h and 10 h, respectively, for both cultures. The frequency of reversion of the individual protoplasts was about 80%. The authors are thankful to Dr. A. Kama, Dr. K. Joseph, and Dr. N.G. Karanth for helpful suggestions and critical comments, and to Dr. B.L. Amla for constant encouragement. P.K.R. Kumar is grateful toCSIR, New Delhi, for the award of a research fellow-ship.