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Identification of the chemical groups involved in the binding of periodate-oxidized NADP+ to 6-phosphogluconate dehydrogenase. |
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| Authors: | F Dallocchio R Negrini M Signorini M Rippa |
| Abstract: | Periodate-oxidized NADP+ binds specifically and reversibly to the NADP+ binding site of 6-phosphogluconate dehydrogenase (EC 1.1.1.44) from Candida utilis. The inhibition can be stabilized by reduction with sodium borohydride. It has been shown that an aldehydic group of the inhibitor forms a Schiff base with a lysine residue of the enzyme. |
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