Prednisolone-Loaded PLGA Microspheres. <Emphasis Type="BoldItalic">In Vitro</Emphasis> Characterization and <Emphasis Type="BoldItalic">In Vivo</Emphasis> Application in Adjuvant-Induced Arthritis in Mice |
| |
Authors: | Khaled A Khaled Hatem A Sarhan Mohamed Abbas Ibrahim Azza H Ali Youssef W Naguib |
| |
Institution: | (1) Department of Pharmaceutics, Faculty of Pharmacy, Minia University, Minia, Egypt;(2) Department of Pharmaceutics, Faculty of Pharmacy, King Saud University, Riyadh, 11451, Kingdom of Saudi Arabia;(3) Department of Histology, Faculty of Medicine, Minia University, Minia, Egypt; |
| |
Abstract: | This study aimed at preparation of a sustained-release steroidal treatment for chronic inflammatory conditions, such as rheumatoid
arthritis. To achieve such a goal, biodegradable poly-lactide-co-glycolide prednisolone-loaded microspheres were prepared
using o/w emulsion solvent evaporation method. Formulation parameters were adjusted so as to optimize the microsphere characteristics.
The prepared microspheres exhibited smooth and intact surfaces, with average size range not exceeding 65 μm. The encapsulation
efficiency percent of most microsphere formulations fell within the range of 25–68%. Drug release from these microspheres
took place over 4 weeks, with near-to-zero-order patterns. Two successful formulations were chosen for the treatment of unilateral
arthritis, induced in mice using Freund's complete adjuvant (FCA). Inflammatory signs of adjuvant arthritis included severe
swelling of the FCA-injected limbs, in addition to many histopathological lesions. These included inflammatory cell infiltration,
synovial hyperplasia, cartilage, and bone erosion. Parenteral administration of the selected formulae dramatically reduced
the swelling of the FCA-injected limbs. In addition, histological examination revealed that the microsphere treatment protocol
efficiently protected cartilages and bones of mice, injected with FCA initial and booster doses, from erosion. These results
could not be achieved by a single prednisolone dose of 5 mg/kg. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|