Synthesis of the herpes simplex virus type 1 trans-activator Vmw65 in insect cells using a baculovirus vector |
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Authors: | John P. Capone Geoffrey Werstuck |
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Affiliation: | (1) Department of Biochemistry, McMaster University, 1200 Main Street West, L8N 3Z5 Hamilton, Ontario, Canada |
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Abstract: | Vmw65, the Herpes Simplex Virus trans-activator of immediate-early genes, was expressed in insect cells using a recombinant baculovirus expression vector and partially purified. Insect cell-derived Vmw65 was shown to be indistinguishable from authentic Vmw65 present in purified HSV-1 virions based on electrophoretic mobility, immunoreactivity with a monoclonal antibody, and ability to interact with cellular factors to form a protein/DNA complex with oligonucleotides containing a TAATGARAT element.Abbreviations AcNPV Autographica californica nuclear polyhedrosis virus - HSV Herpes Simplex Virus - IE Immediate Early - moi multiplicity of infection - Sf9 Spodoptera frugiperda cells |
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Keywords: | baculovirus expression system Herpes Simplex Virus trans-activator protein DNA complex formation |
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