On-line determination of intracellular beta-galactosidase activity in recombinant Escherichia coli using flow injection analysis (FIA). |
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| Authors: | H A Kracke-Helm L Brandes B Hitzmann U Rinas K Schügerl |
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| Affiliation: | Institut für Technische Chemie, Universit?t Hannover, F.R.G. |
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| Abstract: | A flow injection analysis (FIA) system was developed for the determination of cytoplasmic beta-galactosidase activity in recombinant Escherichia coli. The FIA system and its application for on-line monitoring of beta-galactosidase production during cultivation of recombinant E. coli in a 60-l airlift tower loop reactor is described. The results demonstrate that an FIA assay in conjunction with a cell disintegration step can be applied successfully for on-line monitoring of intracellular protein formation. |
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