Regulation of anaerobic carbon monoxide oxidation activity in Rhodocyclus gelatinosus |
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Authors: | James E. Champine Robert L. Uffen |
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Affiliation: | Department of Microbiology and Public Health, Michigan State University, East Lansing, MI, U.S.A. |
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Abstract: | Rhodocyclus gelatinosus grows anaerobically at the expense of carbon monoxide (CO). The CO-oxidation system was substrate-induced and in CO/light, cells grew at an exponential rate with ever increasing amounts of CO:MV oxidoreductase activity (the measure of CO oxidation). Once strain 1 reached a high cell density, the concentration of CO became limiting and gas oxidation activity suddenly decreased. Cell growth continued unaffected. To help explain this, it appeared that strain 1 variably used both CO oxidation and photometabolism to support growth in CO/light. Light intensity determined the upper limit of amounts of CO:MV oxidoreductase in a culture, while intermediate amounts were regulated by CO concentration. Thus, in darkness, cells produced the maximum CO oxidation activity, whereas in growth-saturating light, the minimum limit occurred. The lower the levels of CO:MV oxidoreductase in cells, the greater the content of bacteriochlorophyll. In this manner, strain 1 grew with a generation time of 6.7 independent of light intensity. |
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Keywords: | Rhodocyclus gelatinosus Carbon monoxide Light Carbon monoxide dehydrogenase |
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