利用等位酶对油松无性系种子进行父本分析

利用水平淀粉凝胶电泳8种酶10个位点34个等位基因，对辽宁兴城油松无性系种子园中17个无性系20个单株的200粒种子作了父本分析。无性系雄花量的多少与授粉果率并不成正比；不同无性系对花粉的接受存在一定的选择性，分析试样单株与周围的无性系并没有构成固定的授粉组合，说明授粉期风向等气象因子，无性系的雌雄花器的构造，受精等对受粉的成败都有作用，种子园结实初期自交现象并不明显，在分析试样中只有16＃，17＃，19＃无性系存在自交现象；种子园内花偻的有效传播一般在半径30m范围内，同时，分析了种子园子代的等位酶变异和空间遗传结构，从另一侧面反映出采种母树周围花粉分布比较均匀，并说明散粉期间风向等气象因子对授粉成败影响较大。

A PATERNITY ANALYSIS OF SEEDS FROM DIFFERENT CLONES IN A PINUS TABULAEFORMIS CARR. SEED ORCHARD

Paternity analysis of a clonal seed orchard of Pinus tabulaeformis Carr. was conducted by means of allozyme markers. Multilocus allozyme genotypes at 10 loci of 17 clones were inferred from a sample of at least eight megagametophytes per clone, while the genotypes of the other 32 parental clones were from buds. A total of 200 embryos and endosperms were analyzed, of which the paternity could be accurately identified for just 89 samples. A low degree of selfing was found within the seed orchard, while higher male strobili production did not indicate a higher degree of fertilization success. Half of the allozyme loci were found to deviate from Hardy-Weinberg equilibrium. At the same time, the effective dispersal distance of pollen and spatial genetic structure of the seed orchard population were analyzed by means of spatial autocorrelation. A few Moran's I values for this test in short distance classes were significantly positive. The results indicated that the pattern of spatial genetic structure is very weak in the progeny population.