Search for polythionates in cultures of Chromatium vinosum after sulfide incubation |
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Authors: | Ralf Steudel Gabriele Holdt Pieter T. Visscher Hans van Gemerden |
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Affiliation: | (1) Institut für Anorganische und Analytische Chemie der Technischen Universität Berlin, Sekr. C2, D-1000 Berlin 12, Germany;(2) Department of Microbiology, University of Groningen, Kerklaan 30, NL9751 NN Haren, The Netherlands |
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Abstract: | Cultures of Chromatium vinosum, devoid of sulfur globules, were supplemented with sulfide and incubated under anoxic conditions in the light. The concentrations of sulfide, polysulfides, thiosulfate, polythionates and elemental sulfur (sulfur rings) were monitored for 3 days by ion-chromatography and reversed-phase HPLC. While sulfide disappeared rapidly, thiosulfate and elemental sulfur (S6, S7 S8 rings) were formed. After sulfide depletion, the concentration of thiosulfate decreased fairly rapidly, but elemental sulfur was oxidized very slowly to sulfate. Neither polysulfides (Sx2–), polythionates (SnO62–, n=4–6), nor other polysulfur compounds could be detected, which is in accordance with the fact that sulfide-grown cells were able to oxidize polysulfide without lag. The nature of the intracellular sulfur globules is discussed. |
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Keywords: | Chromatium vinosum Phototrophic bacteria Polysulfides Polythionates Elemental sulfur Sulfur globules Ion chromatography HPLC |
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