Enantioselective synthesis of (S)-2-cyano-2-methylpentanoic acid by nitrilase |
| |
| Authors: | Toyokazu Yoshida Koichi Mitsukura Takuya Mizutani Ryo Nakashima Yasuyo Shimizu Hiroshi Kawabata Toru Nagasawa |
| |
| Institution: | 1. Department of Biomolecular Science, Gifu University, Yanagido 1-1, Gifu, 501-1193, Japan
2. Biotechnology Laboratory, Mitsubishi Chemical Group Science and Technology Research Center, Inc., 1000 Kamoshida-cho, Aoba-ku, Yokohama, 227-8502, Japan
|
| |
| Abstract: | The nitrilase gene of Rhodococcus rhodochrous J1 was expressed in Escherichia coli using the expression vector, pKK223-3. The recombinant E. coli JM109 cells hydrolyzed enantioselectively 2-methyl-2-propylmalononitrile to form (S)-2-cyano-2-methylpentanoic acid (CMPA) with 96 % e.e. Under optimized conditions, 80 g (S)-CMPA l?1 was produced with a molar yield of 97 % at 30 °C after a 24 h without any by-products. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
