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Secretion of RNA by normal and transformed cells |
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| Authors: | G M Kolodny L A Culp L J Rosenthal |
| Affiliation: | 1 Radiology Research Laboratory, Massachusetts General Hospital, Boston, Mass. 02114, USA;2 Infectious Diseases Unit (Department of Medicine) Massachusetts General Hospital, Boston, Mass. 02114, USA;3 Huntington Laboratories of Harvard University, and Massachusetts General Hospital, Boston, Mass. 02114, USA |
| Abstract: | 3T3 and SV-40 transformed 3T3 cells in culture secrete RNA into their culture media. This medium RNA is predominantly 5s in size as measured by sucrose gradient and Sephadex gel filtration. Medium RNA is metabolically stable and is heavily methylated in comparison with other major cytoplasmic species. Analysis of the radioactively labeled methylated bases of medium RNA by paper chromatography after formic acid hydrolysis shows a very simple pattern of two peaks in contrast to the very complex patterns seen in rRNA and tRNA. Mycoplasma and mouse leukemia virus contamination have been excluded. The source of this RNA is discussed. |
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