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酵母菌中SOD复合酶的初步研究
引用本文:王岁楼,张平之,张鑫,何克进. 酵母菌中SOD复合酶的初步研究[J]. 生物学杂志, 2000, 17(4): 26-27
作者姓名:王岁楼  张平之  张鑫  何克进
作者单位:郑州轻工业学院食品与生物工程系,郑州,450002
基金项目:本课题为河南省自然科学基金项目的部分工作(豫科984033300号)
摘    要:对不同酵母菌中SOD等抗氧化酶的活性进行了初步的分析测定,筛选出了一株诸酶活性都较高的菌株(丹宝利面包活性干酵母)。研究了该酵母在不同培养时期SOD等酶少力的变化情况,发现POD、CAT等酶的活性水平SOD活性的变化有密切的相关性。通过比较几种提取方法的效果,认为利用甲苯破壁法提取SOD复合酶具有一定的可行性。

关 键 词:酵母菌 超氧化物歧化酶 过氧化物酶 SOD5复合酶
文章编号:1008-9632(2000)04-0026-02
修稿时间:2000-04-22

A preliminary study on the compound enzyme of superoxide dismutase from yeast
WANG Sui-lou,ZHANG Ping-zhi,ZHANG Xin,HE Ke-jin. A preliminary study on the compound enzyme of superoxide dismutase from yeast[J]. Journal of Biology, 2000, 17(4): 26-27
Authors:WANG Sui-lou  ZHANG Ping-zhi  ZHANG Xin  HE Ke-jin
Abstract:The relation of superoxide dismutase(SOD) with peroxidase (POD) and catalase(CAT) is simply studied by active dry yeast.Under the different period of culture time,the activities of POD and CAT have close relation with that of SOD.During log-growth phase,the activity of SOD increases with culture time,and at the same time the activities of the other two enzymes also increase.After the 1og-growth phase,the SOD activity decreases slightly,and the other two enzymes have similar characteristics.We call the enzyme preparation containing SOD,POD and CAT etc.As the compound enzyme of SOD,and therefore crude extract of SOD from yeast belonge to the compound enzyme of SOD.
Keywords:yeast  superoxide dismutase(SOD)  peroxidase(POD)  catalase(CAT)  the compound enzyme of SOD.0
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