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Cloning of a mercuric ion-resistance operon intoEscherichia coli 5k using the mini-plasmid technique |
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| Authors: | A M A Nascimento M O Azevedo S Astolfi-Filho E Chartone-Souza |
| Institution: | (1) Departamento de Biologia Geral, UFMG, Caixa Postal 2486, Belo Horizonte, 31270, MG, Brazil;(2) Departamento de Biologia Celular, UnB, 70000 Brasília, DF, Brazil |
| Abstract: | Summary Mercury resistant transformant clone ofEscherichia coli 5K bearing the recombinant mini-plasmid pHg (13 MDa) was obtained after treatment of the conjugative 60 MDa R plasmid pBH100 (from wild strainE. coli) withPstI followed by fragment ligation (ligase). |
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