电磁辐射对原代培养海马神经元的损伤效应及其机制探讨

研究X带高功率微波、S带高功率微波及电磁脉冲辐射对原代培养海马神经元的损伤效应并探讨其机制。通过体外培养原代海马神经元,建立电磁波辐照细胞模型。采用Annexin V-PI双标记、流式细胞术检测细胞凋亡与坏死,原子力显微镜检测细胞膜表面形态,Fluo-3-AM荧光探针负载、激光扫描共聚焦显微镜测定胞内Ca2 ]i。结果表明,辐射后海马神经元凋亡与坏死均增加,其中坏死增加明显;细胞膜表面粗糙度加大,膜穿孔增多;胞内Ca2 ]i明显升高。且以上变化均以X带高功率微波组最重,S带高功率微波组次之,电磁脉冲组最轻。提示细胞膜穿孔增多,膜通透性增加,导致胞外Ca2 内流增加,甚至胞内钙超载是辐射致海马神经元凋亡与坏死的机制之一;三种电磁辐射对海马神经元的损伤程度与照射频率呈正相关。

Injury mechanism of primary cultured hippocampus neuron by electromagnetic radiation

To investigate the injury mechanism of primary cultured hippocampus neuron exposed to X-band high power microwave,S-band high power microwave and electromagnetic pulse radiation,primary hippocampus neurons were cultured in vitro,and cell model of electromagnetic radiation was established.Annexin V-PI double labeling and flow cytometry were applied to detect neurons apoptosis and death;Atomic force microscope was used to detect the surface appearance of cellular membrane;Fluo-3-AM fluorescent probe load and laser scanning confocal microscopy were employed to measure Ca2 ]i in neurons.The results indicated that,after radiation,the apoptosis and death rate of hippocampus neurons both increased,and the death rate increased more significantly;The surface roughness and perforation of cellular membrane increased;Ca2 ]i in neurons increased significantly as well.All the changes above showed an attenuating trend in the radiation groups as X-HPM?S-HPM?EMP.It was suggested that perforation increased membrane permeability,and then increased the inflow of extracellular Ca2 ,even resulted in intracellular calcium overload.All these may play an important role in the mechanism of hippocampus neuron apoptosis and death induced by radiation.The injury degree of hippocampus neurons by the three kind of electromagnetic radiation showed a direct correlation with radiation frequency.