Structural feature of sorghum chloroplast psbA gene and regulation effects of its 5'-noncoding region* |
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Authors: | WU Naihu FANG Xiaohua SHI Xiaomei ZHANG Xiaowu ZHOU Li HUANG Meijuan |
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Institution: | (1) Institute of Developmental Biology, Chinese Academy of Sciences, 100080 Beijing, China;(2) School of Life Sciences, Peking University, 100871 Beijing, China |
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Abstract: | Comparative analysis reveals remarkahle homology between the sequences of bothpsbA gene nucleotides and the inferred amino acids of sorghum, a C4 plant, and those of rice, a C3 plant. The 5′-noncoding region of sorghumpsbA gene contains the conservative promoter elements, “—35” element and “—10” element, like the prokaryote and the promoter element,
TATA box, like the eukaryote. As compared with that of the rice, an extra sequence of 7 bp is found in the leader sequence
of the mRNA in the former. Using anin vitro system, it has been demonstrated that protein factor exists in sorghum chloroplast protein extract which specifically hinds
to the 5′-noncoding region ofpsbA gene. Measurement of the expression of luciferase shows a 2–5 time greater reaction of the expression plasmids pALqs which
contain leader region of sorghumpsbA gene than that of the expression plasmids pALqr which contain leader region of ricepsbA gene inE. coli.
Project supported by the Chinese National “863” and “973” Projects |
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