Cooperative erythropoietic assay of several steroid metabolites in polycythemic mice |
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Authors: | James W. Fisher John W. Adamson James F. Camiscoli Walter Fried Albert S. Gordon John Schooley Esmail Zanjani |
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Affiliation: | Department of Pharmacology Tulane University School of Medicine 1430 Tulane Avenue New Orleans, Louisiana 70112, USA |
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Abstract: | A blinded cooperative assay of several androstane and pregnane steroid metabolites has been carried out in order to determine whether 5β-H derivatives are as active as testosterone in stimulating in vivo erythropoiesis. The steroids tested were: testosterone, 5-dihvdrotestosterone, 5β-dihydrotestosterone, 5β-pregnane-3,20-dione, 3-dihydroxy-5β-pregnàne-11,20-dione and 3β-hydroxy-5β-pregnan-20-one. The incorporation of radioactive iron into newly formed red cells in exhypoxic polycythemic mice was used to compare the effects of the steroids. Testosterone and 5-dihydrotestosterone both produced significant increases in 59Fe incorporation. 5β-dihydrotestosterone, 5β-pregnane-3,20-dione, 3-hydroxy-5β-pregnane-11,20-dione and 3β-hydroxy-5β-pregnan-20-one were all devoid of significant erythropoietic activity in polycythemic mice in almost all instances. Thus, under the conditions chosen, this study failed to demonstrate that 5β-steroids increase radioactive iron incorporation in red cells of exhypoxic polycythemic mice. |
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