Mutation detection using ENDO1: Application to disease diagnostics in humans and TILLING and Eco-TILLING in plants |
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| Authors: | Karine Triques Elodie Piednoir Marion Dalmais Julien Schmidt Christine Le Signor Mark Sharkey Michel Caboche Bénédicte Sturbois Abdelhafid Bendahmane |
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| Affiliation: | 1.Unité Mixte de Recherche en Génomique Végétale,Evry,France;2.University of Massachusetts Medical School,Worcester,USA;3.Unité Mixte de Recherche en Génétique et Ecophysiologie des Légumineuses, Domaine d'Epoisses,Bretenières,France |
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| Abstract: | Background Most enzymatic mutation detection methods are based on the cleavage of heteroduplex DNA by a mismatch-specific endonuclease at mismatch sites and the analysis of the digestion product on a DNA sequencer. Important limitations of these methods are the availability of a mismatch-specific endonuclease, their sensitivity in detecting one allele in pool of DNA, the cost of the analysis and the ease by which the technique could be implemented in a standard molecular biology laboratory. |
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