Reliable identification of Prevotella and Butyrivibrio spp. from rumen by fatty acid methyl ester profiles |
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Authors: | Logar R M Zorec M Kopecný J |
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Institution: | (1) Zootechnical Department, Biotechnical Faculty, University of Ljubljana, 1230 Domžale, Slovenia;(2) Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic, 10400 Prague 10, Czechia |
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Abstract: | Data for bacterial identification were provided by culturing anaerobic bacteria under standardized conditions followed by
extraction and methylation of cellular long-chain fatty acids and gas chromatographic analysis. The databases of fatty acid
methyl ester (FAMEs) profiles for two predominant ruminal genera,Prevotella andButyrivibrio, were created. Major long-chain cellular fatty acids found in the 23 analyzedPrevotella strains were 15:0 (anteiso), 15:0, 15:0 (iso) and 16:0. The strains ofPrevotella could be well identified on species level by the characteristic ratios among major fatty acids and by acids unique fatty
for each species. The 45Butyrivibrio strains were grouped into 4 major and 2 minor groups according to FAMEs profiles. The major fatty acids for the bulk of theButyrivibrio strains were 14:0, 15:1, 16:0 and 16:0 (iso). This groups corresponded to those based on 16S rDNA sequences. |
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